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bl21 competent cells  (New England Biolabs)


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    Structured Review

    New England Biolabs bl21 competent cells
    Bl21 Competent Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 880 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+bl21+competent+cells/BL21+Comp+E%2Ecoli/pmc13155313-202-6-9
    Average 96 stars, based on 880 article reviews
    bl21 competent cells - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: The reticulocyte restriction: invasion ligand RBP1a of Plasmodium vivax targets human TfR1, prohibitin-2, and basigin
    Article Snippet: .. Recombinant plasmids pET-28a(+)PvRBP1a158-650LTID and pET-28a(+)LTID were obtained through gene synthesis services by Twist Bioscience (San Francisco, CA, USA) and transformed into E. coli BL21 competent cells (New England Biolabs). .. Recombinant plasmids pET-28a(+)PvRBP1a158-650LTID and pET-28a(+)LTID were obtained through gene synthesis services by Twist Bioscience (San Francisco, CA, USA) and transformed into E. coli BL21 competent cells (New England Biolabs).

    Transformation Assay:

    Article Title: The reticulocyte restriction: invasion ligand RBP1a of Plasmodium vivax targets human TfR1, prohibitin-2, and basigin
    Article Snippet: .. Recombinant plasmids pET-28a(+)PvRBP1a158-650LTID and pET-28a(+)LTID were obtained through gene synthesis services by Twist Bioscience (San Francisco, CA, USA) and transformed into E. coli BL21 competent cells (New England Biolabs). .. Recombinant plasmids pET-28a(+)PvRBP1a158-650LTID and pET-28a(+)LTID were obtained through gene synthesis services by Twist Bioscience (San Francisco, CA, USA) and transformed into E. coli BL21 competent cells (New England Biolabs).

    Article Title: LYPLAL1 Rare Loss-of-Function Variants in Humans and Deletion in Human Hepatoma Cells Protect Against MASLD.
    Article Snippet: .. LYPLAL1 cDNA was cloned into pGEX-4T-1 expression vector (Addgene, #27458001) and transformed into E. coli BL21 competent cells (New England Biolabs, #C2530H). ..

    Article Title: The energetic and allosteric landscape for KRAS inhibition
    Article Snippet: KRAS residues 1–169 fused to an N-terminal His 6 tag and a TEV protease cleavage site was cloned into a pCoofy31 vector, and variants were generated by using the Q5 site-directed mutagenesis kit (New England Biolabs). .. Vectors were transformed into E. coli BL21 competent cells (NEB), and single colonies were picked to grow overnight pre-cultures to saturation in Luria-Bertani broth (LB) containing 33 μg ml −1 kanamycin. ..

    Article Title: TIR1-produced cAMP as a second messenger in transcriptional auxin signalling
    Article Snippet: .. Coding domain sequences of axr2 (P88L) and axr3 (P88L) were cloned into pGEX4T-1 vector, and the resultant plasmids confirmed by sequencing were transformed into E. coli BL21 competent cells (NEB, catalogue no. C2530H) for protein purification. ..

    Article Title: Transmembrane coupling of liquid-like protein condensates.
    Article Snippet: .. The pGEX4T2-GST-ThrombinENTH-RGG plasmidwas transformed into E. coli BL21 competent cells (New England Biolabs #C2530). ..

    Article Title: LYPLAL1 rare loss-of-function variants in humans and deletion in human hepatoma cells protect against MASLD
    Article Snippet: .. LYPLAL1 cDNA was cloned into pGEX-4T-1 expression vector (Addgene, #27458001) and transformed into E. coli BL21 competent cells (New England Biolabs, #C2530H). ..

    Article Title: The energetic and allosteric landscape for KRAS inhibition.
    Article Snippet: Recombinant protein sample preparation KRAS residues 1–169 fused to an N-terminal His6 tag and a TEV protease cleavage site was cloned into a pCoofy31 vector, and variants were generated by using the Q5 site-directed mutagenesis kit (New England Biolabs). .. Vectors were transformed into E. coli BL21 competent cells (NEB), and single colonies were picked to grow overnight pre-cultures to saturation in Luria-Bertani broth (LB) containing 33 μg ml−1 kanamycin. ..

    Article Title: TIR1-produced cAMP as a second messenger in transcriptional auxin signalling.
    Article Snippet: .. Coding domain sequences of axr2 (P88L) and axr3 (P88L) were cloned into pGEX4T-1 vector, and the resultant plasmids confirmed by sequencing were transformed into E. coli BL21 competent cells (NEB, catalogue no. C2530H) for protein purification. ..

    Clone Assay:

    Article Title: LYPLAL1 Rare Loss-of-Function Variants in Humans and Deletion in Human Hepatoma Cells Protect Against MASLD.
    Article Snippet: .. LYPLAL1 cDNA was cloned into pGEX-4T-1 expression vector (Addgene, #27458001) and transformed into E. coli BL21 competent cells (New England Biolabs, #C2530H). ..

    Article Title: TIR1-produced cAMP as a second messenger in transcriptional auxin signalling
    Article Snippet: .. Coding domain sequences of axr2 (P88L) and axr3 (P88L) were cloned into pGEX4T-1 vector, and the resultant plasmids confirmed by sequencing were transformed into E. coli BL21 competent cells (NEB, catalogue no. C2530H) for protein purification. ..

    Article Title: LYPLAL1 rare loss-of-function variants in humans and deletion in human hepatoma cells protect against MASLD
    Article Snippet: .. LYPLAL1 cDNA was cloned into pGEX-4T-1 expression vector (Addgene, #27458001) and transformed into E. coli BL21 competent cells (New England Biolabs, #C2530H). ..

    Article Title: TIR1-produced cAMP as a second messenger in transcriptional auxin signalling.
    Article Snippet: .. Coding domain sequences of axr2 (P88L) and axr3 (P88L) were cloned into pGEX4T-1 vector, and the resultant plasmids confirmed by sequencing were transformed into E. coli BL21 competent cells (NEB, catalogue no. C2530H) for protein purification. ..

    Expressing:

    Article Title: LYPLAL1 Rare Loss-of-Function Variants in Humans and Deletion in Human Hepatoma Cells Protect Against MASLD.
    Article Snippet: .. LYPLAL1 cDNA was cloned into pGEX-4T-1 expression vector (Addgene, #27458001) and transformed into E. coli BL21 competent cells (New England Biolabs, #C2530H). ..

    Article Title: LYPLAL1 rare loss-of-function variants in humans and deletion in human hepatoma cells protect against MASLD
    Article Snippet: .. LYPLAL1 cDNA was cloned into pGEX-4T-1 expression vector (Addgene, #27458001) and transformed into E. coli BL21 competent cells (New England Biolabs, #C2530H). ..

    Plasmid Preparation:

    Article Title: LYPLAL1 Rare Loss-of-Function Variants in Humans and Deletion in Human Hepatoma Cells Protect Against MASLD.
    Article Snippet: .. LYPLAL1 cDNA was cloned into pGEX-4T-1 expression vector (Addgene, #27458001) and transformed into E. coli BL21 competent cells (New England Biolabs, #C2530H). ..

    Article Title: LYPLAL1 rare loss-of-function variants in humans and deletion in human hepatoma cells protect against MASLD
    Article Snippet: .. LYPLAL1 cDNA was cloned into pGEX-4T-1 expression vector (Addgene, #27458001) and transformed into E. coli BL21 competent cells (New England Biolabs, #C2530H). ..

    Sequencing:

    Article Title: TIR1-produced cAMP as a second messenger in transcriptional auxin signalling
    Article Snippet: .. Coding domain sequences of axr2 (P88L) and axr3 (P88L) were cloned into pGEX4T-1 vector, and the resultant plasmids confirmed by sequencing were transformed into E. coli BL21 competent cells (NEB, catalogue no. C2530H) for protein purification. ..

    Article Title: TIR1-produced cAMP as a second messenger in transcriptional auxin signalling.
    Article Snippet: .. Coding domain sequences of axr2 (P88L) and axr3 (P88L) were cloned into pGEX4T-1 vector, and the resultant plasmids confirmed by sequencing were transformed into E. coli BL21 competent cells (NEB, catalogue no. C2530H) for protein purification. ..

    Protein Purification:

    Article Title: TIR1-produced cAMP as a second messenger in transcriptional auxin signalling
    Article Snippet: .. Coding domain sequences of axr2 (P88L) and axr3 (P88L) were cloned into pGEX4T-1 vector, and the resultant plasmids confirmed by sequencing were transformed into E. coli BL21 competent cells (NEB, catalogue no. C2530H) for protein purification. ..

    Article Title: TIR1-produced cAMP as a second messenger in transcriptional auxin signalling.
    Article Snippet: .. Coding domain sequences of axr2 (P88L) and axr3 (P88L) were cloned into pGEX4T-1 vector, and the resultant plasmids confirmed by sequencing were transformed into E. coli BL21 competent cells (NEB, catalogue no. C2530H) for protein purification. ..



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    Construction of engineered E. coli strains and protein purification. (A) PCR identification of positive E. coli clones. Lanes 1–3 correspond to BL21-pET28-ST-PCV2 Cap, BL21-pET28-ST-PCV3 Cap, and BL21-pET28-SC-mi3, respectively. (B–D) Expression and purification of recombinant proteins ST-PCV2 Cap, ST-PCV3 Cap, and SC-mi3, respectively. Lanes 1–6 represent whole bacterial lysate, pellet after sonication, supernatant after sonication, Ni column flow-through, wash fraction, and purified protein, respectively. (E) Western blot validation of antigen proteins. Lanes 1, 2 correspond to ST-PCV2 Cap and ST-PCV3 Cap, respectively. (F) Endotoxin removal from recombinant protein solutions. Color intensity represents endotoxin content; numbers indicate endotoxin content (EU/mL).
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    Construction of engineered E. coli strains and protein purification. (A) PCR identification of positive E. coli clones. Lanes 1–3 correspond to BL21-pET28-ST-PCV2 Cap, BL21-pET28-ST-PCV3 Cap, and BL21-pET28-SC-mi3, respectively. (B–D) Expression and purification of recombinant proteins ST-PCV2 Cap, ST-PCV3 Cap, and SC-mi3, respectively. Lanes 1–6 represent whole bacterial lysate, pellet after sonication, supernatant after sonication, Ni column flow-through, wash fraction, and purified protein, respectively. (E) Western blot validation of antigen proteins. Lanes 1, 2 correspond to ST-PCV2 Cap and ST-PCV3 Cap, respectively. (F) Endotoxin removal from recombinant protein solutions. Color intensity represents endotoxin content; numbers indicate endotoxin content (EU/mL).
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    New England Biolabs e coli bl21 de3 cells
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    Image Search Results


    Construction of engineered E. coli strains and protein purification. (A) PCR identification of positive E. coli clones. Lanes 1–3 correspond to BL21-pET28-ST-PCV2 Cap, BL21-pET28-ST-PCV3 Cap, and BL21-pET28-SC-mi3, respectively. (B–D) Expression and purification of recombinant proteins ST-PCV2 Cap, ST-PCV3 Cap, and SC-mi3, respectively. Lanes 1–6 represent whole bacterial lysate, pellet after sonication, supernatant after sonication, Ni column flow-through, wash fraction, and purified protein, respectively. (E) Western blot validation of antigen proteins. Lanes 1, 2 correspond to ST-PCV2 Cap and ST-PCV3 Cap, respectively. (F) Endotoxin removal from recombinant protein solutions. Color intensity represents endotoxin content; numbers indicate endotoxin content (EU/mL).

    Journal: Frontiers in Veterinary Science

    Article Title: Construction and immunogenicity evaluation of a bivalent nanoparticle based on mi3 displaying porcine circovirus type 2 and type 3 capsid proteins

    doi: 10.3389/fvets.2026.1862938

    Figure Lengend Snippet: Construction of engineered E. coli strains and protein purification. (A) PCR identification of positive E. coli clones. Lanes 1–3 correspond to BL21-pET28-ST-PCV2 Cap, BL21-pET28-ST-PCV3 Cap, and BL21-pET28-SC-mi3, respectively. (B–D) Expression and purification of recombinant proteins ST-PCV2 Cap, ST-PCV3 Cap, and SC-mi3, respectively. Lanes 1–6 represent whole bacterial lysate, pellet after sonication, supernatant after sonication, Ni column flow-through, wash fraction, and purified protein, respectively. (E) Western blot validation of antigen proteins. Lanes 1, 2 correspond to ST-PCV2 Cap and ST-PCV3 Cap, respectively. (F) Endotoxin removal from recombinant protein solutions. Color intensity represents endotoxin content; numbers indicate endotoxin content (EU/mL).

    Article Snippet: The pET28a(+) plasmid for recombinant protein expression and BL21(DE3) E. coli competent cells were purchased from Sangon Biotech (Shanghai) Co., Ltd.

    Techniques: Protein Purification, Clone Assay, Expressing, Purification, Recombinant, Sonication, Western Blot, Biomarker Discovery

    The plasmid pET16b containing the Costa Rica Fluc gene was used to transform E. coli BL21 (DE3) and the colonies were transferred to nitrocellulose, induced with IPTG and screened with luciferin. Bioluminescence was detected on a PhotonIMAGER Optima and the bioluminescent intensity displayed from blue (low intensity) to red (high intensity). The bioluminescent activity of the plated transformants from the primary screen over a 20 second intergral is shown in (A). In (B) randomly picked colonies from the primary screen, were screened for bioluminescent activity. Images were produced using M3 Vision software and the intensity scaling between (A) and (B) is not directly comparable.

    Journal: bioRxiv

    Article Title: Bioprospecting Novel Luciferase Genes from Museum Coleoptera

    doi: 10.64898/2026.04.21.719859

    Figure Lengend Snippet: The plasmid pET16b containing the Costa Rica Fluc gene was used to transform E. coli BL21 (DE3) and the colonies were transferred to nitrocellulose, induced with IPTG and screened with luciferin. Bioluminescence was detected on a PhotonIMAGER Optima and the bioluminescent intensity displayed from blue (low intensity) to red (high intensity). The bioluminescent activity of the plated transformants from the primary screen over a 20 second intergral is shown in (A). In (B) randomly picked colonies from the primary screen, were screened for bioluminescent activity. Images were produced using M3 Vision software and the intensity scaling between (A) and (B) is not directly comparable.

    Article Snippet: A gene encoding the proposed Costa Rica Fluc was designed as codon optimised for E. coli and was synthesized by IDT and incorporated into a pET16b vector (Sigma-Aldrich) to enable transformation of E. coli BL21 (DE3) competent cells (Fisher Scientific).

    Techniques: Plasmid Preparation, Activity Assay, Produced, Software